小松　雅明 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.med.niigata-u.ac.jp/bc1/index.html" target="_blank">Lab HP (Japanese)</A> B6.Cg-Atg7<tm1Tchi> B6.Cg-Atg7<tm1Tchi> Atg7, autophagy-related 7 gene floxed mice. A targeting vector was constructed by insertion of a loxP site within introns 13 and 14. Exon 14 was fused to a cDNA fragment encoded by exons 15, 16 and 17 and polyA after the stop codon. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Conditional Atg7 deficient mice can be generated by crossing with tissue-specific Cre mice. Polyoma enhancer/herpes simplex virus thymidine kinase gene (MC1) promoter, E. coli Neomycin resistance gene, Phage P1 loxP sites, Mouse Atg7 genomic DNA <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jun_2012_mm" target="_blank">Mouse of the Month Jun 2012</A>, <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/oct_2016_mm" target="_blank">Mouse of the Month Oct 2016</A> RBRC02759 true The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Blol., 169, 425-434 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE. <a href='https://brc.riken.jp/mus/pcr02759'>Genotyping protocol -PCR-</a> TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] B（1〜3か月） Developed by Masaaki Komatsu and Tomoyuki Chiba, Tokyo Metropolitan Institute of Medical Science. The targeting construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mice were backcrossed to C57BL/6 over 5 times. C57BL/6-Atg7 flox/flox C57BL/6-Atg7 flox/flox B (1-3 months) Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile. Masaaki KOMATSU 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Cell Blol., 169, 425-434 (2005).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>公表を前提とした学術研究に限る。営利機関の使用にあたっては事前に寄託者からの許可を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。